Recombinant Human IL-6/Interleukin-6 Protein

Specifications

Catalog number

PKSH033611-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

Abbreviation

IL-6; Interleukin-6

Synonyms

IFNB; Interleukin HP; Interferon Beta; B-Cell Stimulatory Factor; IFN-Beta; B-cell hybridoma growth factor; B-Cell Stimulatory Factor 2; BSF2; BSF-2; CDF; CTL Differentiation Factor; HGF; HSF; Hybridoma Growth Factor; IFN-Beta-2; IL-6; Interferon Beta-2; Interleukin 6; Interleukin HP-1; Interleukin-6; IL6; IFNB2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; Hepatocyte stimulatory factor; Hybridoma growth factor Interferon beta-2; IL 6; Interferon beta 2; Interleukin 6 (interferon beta 2) ; Interleukin BSF 2; Interleukin-6; IL-6; B-cell hybridoma growth factor; Interleukin HP-1

UniProt

P05231

Accession Number

P05231

Expression System

E.coli

Tag

C-His

Sequence

Val30-Met212

Applications

Cell culture

Field of Research

Immunology, Neuroscience, Cancer, Metabolism

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

505 x 108 IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is 850

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.8 kDa

Observed Molecular Weight

22 kDa

Species

Human