Recombinant Human Interleukin-33/IL-33 Protein

Specifications

Catalog number

PKSH033616-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils; Peyers' s patches; and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus; and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment; corresponding to mature IL-33; binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2; activates NF-kappaB and MAP kinases; and drives production of T (H) 2-associated cytokines from in vitro polarized T (H) 2 cells. In vivo; IL-33 induces the expression of IL-4; IL-5; and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.

Abbreviation

Interleukin-33; IL-33

Synonyms

DVS; IL1F; Interleukin; C9orf; IL-1F; IL33; C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; DVS27 related protein; IL-1F11; IL-33; Interleukin-1 family member 11; Interleukin-33; Nuclear factor from high endothelial venules; NFHEV; CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; IL 1F11; IL 33; Interleukin 1 family member 11; Interleukin 33; INTERLEUKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; Interleukin-33 (109-270) ; NF HEV; Nuclear factor for high endothelial venules; OTTHUMP00000021041; RP11 575C20.2

UniProt

O95760

Accession Number

O95760

Expression System

E.coli

Tag

None

Sequence

Ser112-Thr270

Field of Research

Cardiovascular; immunology

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.1 kDa

Observed Molecular Weight

18 kDa

Species

Human