Recombinant Human IL10-RA/IL-10 Rα Protein (His Tag)

Specifications

Catalog number

PKSH033632-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-10 Receptor alpha (IL-10Rα) is a transmembrane glycoprotein member of the class II cytokine receptor family. Mature human IL-10 Rα consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain. Within the ECD, human IL-10 Rα shares 59% aa sequence identity with mouse and rat IL-10Rα. IL-10 Rα is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10Rα is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ. Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection. Polymorphisms of human IL-10 Rα may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL10.

Abbreviation

IL10-RA; IL-10 Rα

Synonyms

IL-10R subunit; Interleukin-10 receptor subunit; IL-10R; IL10RA; CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10RA; Interleukin-10 receptor subunit 1; Interleukin-10 receptor subunit alpha; IL-10R1; IL-10Ra; CDw210a antigen; HIL 10R; HIL10R; I10R1; IL 10R; IL 10R 1; IL 10R A; IL 10R1; IL10R1; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain

UniProt

Q13651

Accession Number

Q13651

Expression System

HEK293 Cells

Tag

C-His

Sequence

His22-Asn235

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.2 kDa

Observed Molecular Weight

38-59 kDa

Species

Human