Recombinant Human GDF5/BMP-14 Protein

Specifications

Catalog number

PKSH033660-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Growth Differentiation Factor 5 (GDF-5, BMP-14) is a member of the BMP family of TGFβ superfamily proteins. Human GDF-5, -6, and -7 are a defined subgroup of the BMP family. GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) Nterminal proregion and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-IIor Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) . GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis. Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis.

Abbreviation

GDF5; BMP-14

Synonyms

BMP-14; Bone morphogenetic protein 14; CDMP-1; CDMP1; Cartilage-derived morphogenetic protein 1; GDF-5; Growth/differentiation factor 5; LAP-4; LPS-associated protein 4; Lipopolysaccharide-associated protein 4; Radotermin

UniProt

P43026

Accession Number

P43026

Expression System

E.coli

Tag

C-His

Sequence

Ala382-Arg501

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Stem cells

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.5 kDa

Observed Molecular Weight

18 kDa

Species

Human