Recombinant Human G-CSF/CSF3 Protein

Specifications

Catalog number

PKSH033663-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

Abbreviation

G-CSF; CSF3

Synonyms

C17orf33; CSF3; Filgrastim; G-CSF; GCSF; Granulocyte Colony-Stimulating Factor; Lenograstim; Pluripoietin

UniProt

P09919

Accession Number

P09919-2

Expression System

E.coli

Tag

None

Sequence

Thr31-Pro204

Field of Research

Immunology, Cancer

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.8 kDa

Observed Molecular Weight

16 kDa

Species

Human