Recombinant Human EphA1 Protein (His Tag)

Specifications

Catalog number

PKSH033690-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-A receptor 1/EphA1 is a glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. EphA1 can form pH sensitive cishomodimers on the cell surface. Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation. Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes. EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness.

Abbreviation

EphA1

Synonyms

EPH; EPH tyrosine kinase; EPH tyrosine kinase 1; EPHA1; EPHT; EPHT1; Ephrin type-A receptor 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH; hEpha1

UniProt

P21709

Accession Number

AAI30292.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Lys26-Glu547

Field of Research

Cardiovascular; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.4 kDa

Observed Molecular Weight

70-85 kDa

Species

Human