Recombinant Human Autotaxin/ENPP2 Protein (aa 36-863, His Tag)

Specifications

Catalog number

PKSH033693-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain.Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

Abbreviation

Autotaxin; ENPP2

Synonyms

ATX; ATX-X; ATXFLJ26803; Autotaxin; E-NPP 2; EC 3.1.4.39; ENPP2; LysoPLD; NPP2; PD-IALPHA; PDNP2; PDNP2NPP2; autotaxin-t; ectonucleotide pyrophosphatase/phosphodiesterase 2

UniProt

Q13822

Accession Number

AAH34961.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala36-Ile863

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

96 kDa

Observed Molecular Weight

100-120 kDa

Species

Human