Recombinant Human CD69 Protein (aa 64-199, His Tag)

Specifications

Catalog number

PKSH033694-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Early Activation Antigen CD69 (CD69) is a type 2 transmembrane glycoprotein in the C-type lectin family. It plays roles in immune cell trafficking, inflammation, T cell memory, and humoral immune responses. CD69 is expressed on the cell surface as an approximately 60 kDa disulfide-linked homodimer. It is found on CD4+ T cells, CD8+ T cells, NK cells, NKT cells, gamma delta cells dendritic cells (DC) and is up-regulated on activated T cells and DC. Ligation of CD69 on DC induces IL2 production, leading to T cell proliferation. CD69 is important for the homing of CD4+ T cells and plasmablasts to the bone marrow but inhibits the migration of dermal DC to draining lymph nodes. It supports the expression of multiple chemokines and chemokine receptors but suppresses the expression of others. It associates with and negatively regulates S1P1 expression on DC and CD4+ T cells, resulting in a decreased chemotactic response to S1P. The direct interaction of CD69 with Galectin-1 contributes to the ability of CD69 to limit Th17 mediated inflamamtion while supporting the differentiation of regulatory T cells.

Abbreviation

CD69

Synonyms

AIM; Activation inducer molecule; BL-AC/P26; C-type lectin domain family 2 member C; CD69; CLEC2C; EA1; Early T-cell activation antigen p60; Early activation antigen CD69; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

UniProt

Q07108

Accession Number

Q07108

Expression System

HEK293 Cells

Tag

N-His

Sequence

Gly64-Lys199

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.9 kDa

Observed Molecular Weight

18-28 kDa

Species

Human