Recombinant Human DKK1/Dkk-1 Protein (His Tag)

Specifications

Catalog number

PKSH033697-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dickkopf-related protein 1 (DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Abbreviation

DKK1; Dkk-1

Synonyms

Dickkopf-1; Dickkopf-related protein 1; Dkk-1; SK

UniProt

O94907

Accession Number

O94907

Expression System

HEK293 Cells

Tag

N-His

Sequence

Thr32-His266

Field of Research

Cardiovascular; Developmental Biology; immunology; Stem cells

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.0 kDa

Observed Molecular Weight

35-50 kDa

Species

Human