Recombinant Human Decorin Protein (Human Cells, His Tag)

Specifications

Catalog number

PKSH033700-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Decorin is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs) . SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) . The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. Alternate splicing of human Decorin generates five isoforms with variable length deletions. Decorin is an N-glycosylated protein that also carries a variablysized hybrid chondroitin/dermatan sulfate chain at Ser34. Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF2; GDF8/Myostatin; TGFβ; and WISP1. It also binds and activates EGF R; ErbB4; and IGFI-R. In vivo; Decorin promotes myoblast differentiation; supports angiogenesis; and inhibits tumor progression.

Abbreviation

Decorin

Synonyms

Bone proteoglycan II; DCN; Decorin; PG-S2; PG40; SLRR1B

UniProt

P07585

Accession Number

P07585

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly17-Lys359

Field of Research

Signal Transduction; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38.8 kDa

Observed Molecular Weight

43-50 kDa

Species

Human