Recombinant Human CTLA4 Protein (mFc Tag)

Specifications

Catalog number

PKSH033703-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytotoxic Tlymphocyte 4 (CTLA-4; CD152) ; is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence; a 126 aa extracellular domain (ECD) with one Ig-like V-type domain; a 21 aa transmembrane (TM) sequence; and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Abbreviation

CTLA4

Synonyms

CD152; CTLA4; Cytotoxic T-Lymphocyte-Associated Protein 4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4

UniProt

P16410

Accession Number

P16410

Expression System

HEK293 Cells

Tag

C-mFc

Sequence

Lys36-Asp161

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.1 kDa

Observed Molecular Weight

45-55 kDa

Species

Human