Recombinant Human Creatine Kinase BB/CKB Protein (His Tag)

Specifications

Catalog number

PKSH033708-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Creatine kinase B-type (CKB) belongs to the ATP:guanido phosphotransferase family. It has dimer of identical or non-identical chains with MM being the major form in skeletal muscle and myocardium. MB exists in myocardium, and BB exists in many tissues, especially brain. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure.

Abbreviation

Creatine Kinase BB; CKB

Synonyms

B-CK; CKB; Creatine Kinase BB

UniProt

P12277

Accession Number

P12277

Expression System

E.coli

Tag

N-His

Sequence

Met1-Lys381

Field of Research

Signal Transduction; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

44.8 kDa

Observed Molecular Weight

50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

Species

Human