Recombinant Human F13a/Factor XIIIa Protein (His Tag)

Specifications

Catalog number

PKSH033713-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.

Abbreviation

F13a; Factor XIIIa

Synonyms

Coagulation Factor XIII A Chain; Coagulation Factor XIIIa; F13A; F13A1; Protein-Glutamine Gamma-Glutamyltransferase A Chain; Transglutaminase A Chain

UniProt

P00488

Accession Number

AAH27963.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly39-Met732

Field of Research

Tags & Cell Markers; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

80.3 kDa

Observed Molecular Weight

80-90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM NaCl, 5% Sucrose, 0.3% Histidine, pH 8.0.

Species

Human