Recombinant Human Coagulation Factor X/F10 Protein (Fc Tag)

Specifications

Catalog number

PKSH033714-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

F10, also known as Coagulation factor X, belongs to the peptidase S1 family that is synthesized as a 488 amino acid (aa) with a signal peptide and a pro region (residues 1-40) . Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41-179) and heavy (residues 235-488) chains linked by a disulfide bond. Coagulation factor X is initially synthesized in the liver. The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. F10 is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Abbreviation

Coagulation Factor X; F10

Synonyms

Coagulation factor 10; FX; FXA; coagulation factor X

UniProt

P00742

Accession Number

P00742

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Asn32-Lys488

Field of Research

Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

78.2 kDa

Observed Molecular Weight

80-120&25 kDa

Formulation

2

Species

Human