Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag)

Specifications

Catalog number

PKSH033722-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein is the receptor that involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. The protein interacts with MARCH8, human herpesvirus 8 MIR2 protein, adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.It contains 1 Ig-like C2-type (immunoglobulin-like) domainand 1 Ig-like V-type (immunoglobulin-like) domain.

Abbreviation

CD86; B7-2

Synonyms

LAB; ETC; FUN; CTLA-4 Counter-Receptor B; Early T-cell costimulatory molecule; CD28LG; B7-2; B7.2; B70; CD28LG2; LAB72; CD86; Activation B7-2 antigen; BU63; CTLA-4 Counter-Receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN-1; T-lymphocyte activation antigen CD86; B7-2; Activation B7-2 antigen 3; B7 2; B72 antigen; B-lymphocyte activation antigen B7-2; B-lymphocyte activation antigen B7-2 2; CD28 antigen ligand 2; CD28 antigen ligand 2 2; Cd28l2; CD86 antigen; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2 2; FUN 1; Ly58; Ly-58; MB7; MB7-2; Membrane glycoprotein; MGC34413; TS/A-2

UniProt

P42081

Accession Number

AAH40261.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala24-Pro247

Applications

BLI

Field of Research

Microbiology; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Human B7-2-His on HIS1K Biosensor, can bind Mouse CTLA-4-Fc with an affinity constant of 5.65 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.7 kDa

Observed Molecular Weight

57-66 kDa

Species

Human