Recombinant Human CD40/TNFRSF5 Protein (mFc Tag)

Specifications

Catalog number

PKSH033724-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells; follicular dendritic cells; dendritic cells; activated monocytes; macrophages; endothelial cells; vascular smooth muscle cells; and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand (CD40L) leads to aggregation of CD40 molecules; which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins (adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching; memory B cell development; and germinal center formation.

Abbreviation

CD40; TNFRSF5

Synonyms

B-Cell Surface Antigen CD40; Bp50; CD40; CD40L Receptor; CDw40; TNFRSF5; Tumor Necrosis Factor Receptor Superfamily member 5

UniProt

P25942

Accession Number

P25942

Expression System

HEK293 Cells

Tag

C-mFc

Sequence

Glu21-Arg193

Field of Research

Cardiovascular; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.7 kDa

Observed Molecular Weight

50-65 kDa

Species

Human