Recombinant Human Complement C5a Protein

Specifications

Catalog number

PKSH033742-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

Abbreviation

Complement C5a

Synonyms

C5Da; C5a; C5a anaphylatoxin; CPAMD4; Complement C5; ECLZB

UniProt

P01031

Accession Number

P01031

Expression System

E.coli

Tag

None

Sequence

Thr678-Arg751

Field of Research

Signal Transduction; Cell biology; immunology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

8.4 kDa

Observed Molecular Weight

12 kDa

Species

Human