Recombinant Human SIGLEC2/CD22 Protein (Fc Tag)

Specifications

Catalog number

PKSH033750-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR) -induced Ca2+ signaling on normal B cells. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface.

Abbreviation

SIGLEC2; CD22

Synonyms

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; CD22 antigenMGC130020; CD22 molecule; SIGLEC-2; SIGLEC2FLJ22814; Siglec-2; T-cell surface antigen Leu-14; sialic acid binding Ig-like lectin 2

UniProt

P20273

Accession Number

P20273

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Asp20-Arg687

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

102.3 kDa

Observed Molecular Weight

120-150 kDa

Species

Human