Recombinant Human BLNK/Ly-57 Protein (His Tag)

Specifications

Catalog number

PKSH033753-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.

Abbreviation

BLNK; Ly-57

Synonyms

B-Cell Adapter Containing a SH2 Domain Protein; B-Cell Adapter Containing a Src Homology 2 Domain Protein; B-Cell Linker Protein; BASH; BLNK; Cytoplasmic Adapter Protein; SLP-65; SLP65; Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa

UniProt

Q8WV28

Accession Number

AAH18906

Expression System

E.coli

Tag

C-His

Sequence

Met1-Ser456

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.5 kDa

Observed Molecular Weight

40-80 kDa

Species

Human