Recombinant Human BCAM Protein (His Tag)

Specifications

Catalog number

PKSH033754-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Basal cell adhesion molecule (BCAM, CD239) is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu) . The ECD of human BCAM contains two Ig-like V-type domains and three Ig-like C2-type domains. It shares 73% aa sequence identity with the ECDs of mouse and rat BCAM. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation. BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle. BCAM is upregulated on carcinomas, particularly ovarian, sarcomas, astrocytomas, and melanomas. It may mediate intracellular signaling. It cooperates with Integrins β1 and αVβ3 as an adhesion receptor for Laminins which contain the α5 chain. The Lutheran isoform is aberrantly phosphorylated in erythroid disorders and can enhance Lamininmediated adhesion of erythrocytes to vascular endothelial cells.

Abbreviation

BCAM

Synonyms

Auberger B antigen; B-CAM cell surface glycoprotein; BCAM; Basal cell adhesion molecule; CD239; F8/G253 antigen; LU; Lutheran antigen; Lutheran blood group glycoprotein; MSK19

UniProt

P50895

Accession Number

P50895

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu32-Ala547

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

72-85 kDa

Species

Human