Recombinant Human B7-H4/VTCN1 Protein (Fc Tag)

Specifications

Catalog number

PKSH033755-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains; a 21 aa transmembrane segment; and a 2 aa cytoplasmic tail. It is widely expressed; including in kidney; liver; lung; pancreas; placenta; prostate; spleen; testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation; proliferation; cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages; plays an important role; together with regulatory T-cells (Treg) ; in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Abbreviation

B7-H4; VTCN1

Synonyms

B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7XPRO1291; B7h.5; B7x; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing ; Vtcn1

UniProt

Q7Z7D3

Accession Number

Q7Z7D3

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Phe29-Ala258

Field of Research

Cell biology; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.5 kDa

Observed Molecular Weight

70-95 kDa

Species

Human