Recombinant Human CD80/B7-1 Protein (mFc Tag)

Specifications

Catalog number

PKSH033784-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cluster of Differentiation 80, also called B7-1, is a member of cell surface immunoglobulin superfamily which plays key, yet distinct roles in the activation of T cells. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. Studies have shown that CTLA-4 binds mostly to CD80. The structure presents two extracellular domains: a membrane distal variable-like domain (IgV) and a membrane proximal Ig constant-like domain (IgC) along with an intracellular domain. Both IgV and IgC consist of anti-parallel beta sandwiches joined by a short linker region. CD80 is mostly expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells.

Abbreviation

CD80; B7-1

Synonyms

Activation B7-1 antigen; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD28LGB7-1 antigen; CD80; LAB7; T-lymphocyte activation antigen CD80

UniProt

P33681

Accession Number

P33681

Expression System

HEK293 Cells

Tag

C-mFc

Sequence

Val35-Leu242

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.5 kDa

Observed Molecular Weight

70-95 kDa

Species

Human