Recombinant Human APRIL/TNFSF13 Protein (His & Flag Tag)

Specifications

Catalog number

PKSH033785-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

APRIL (a proliferation-inducingligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely ofa single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signalthrough the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfateproteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers withBAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells andalso promotes T cell-dependenthumoral responses. APRIL levels are elevated inthe serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratingneutrophils.

Abbreviation

APRIL; TNFSF13

Synonyms

UNQ; PRO; TRDL; ZTNF; UNQ383/PRO; TNF-related death ligand; Tumor necrosis factor ligand superfamily member; TNF- and APOL-related leukocyte expressed ligand; TNFSF; TNFSF13; APRIL; CD256; TALL-2; TALL2; TNLG7B; TRDL-1; UNQ383/PRO715; ZTNF2; A proliferation-inducing ligand; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; Tumor necrosis factor ligand superfamily member 13; UNQ383; PRO715; TALL-2; TNFSF13; A proliferation inducing ligand; member 13; TNF related death ligand; TNF13; TRDL1; Tumor necrosis factor (ligand) superfamily; Tumor necrosis factor like protein ZTNF2; Tumor necrosis factor related death ligand 1

UniProt

O75888

Accession Number

O75888

Expression System

HEK293 Cells

Tag

N-Flag-His

Sequence

Lys112-Leu250

Field of Research

Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.0 kDa

Observed Molecular Weight

60 kDa

Species

Human