Recombinant Human Alpha-enolase/Enolase 1/MPB-1/NNE (C-6His)

Specifications

Catalog number

PKSH033843-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-enolase, also known as MPB-1, NNE, Enolase 1, belongs to the enolase family. Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Isoform MBP-1 interacts with TRAPPC2B. ENO1 interacts with ENO4 and PGAM2. The alpha/alpha homodimer of ENO1 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses and may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. ENO1 also stimulates immunoglobulin production.

Abbreviation

Enolase 1; MPB-1; NNE

Synonyms

Enolase 1; MPB-1; NNE

UniProt

P06733

Accession Number

P06733

Expression System

E.coli

Tag

C-His

Sequence

Met1-Lys434

Field of Research

Signal Transduction; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

48.0 kDa

Observed Molecular Weight

45-55 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM MgSO4, 20% Glycerol, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, pH 7.5.

Species

Human