Recombinant Human Siglec-7/CD328 (C-6His)

Specifications

Catalog number

PKSH033850-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes. It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid. The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

Abbreviation

Siglec-7; CD328

Synonyms

CDw329; Protein FOAP-9; SIGLEC9; Sialic acid-binding Ig-like lectin 9; Siglec-9

UniProt

Q9Y286

Accession Number

Q9Y286

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln19-Leu353

Field of Research

Cardiovascular; Neuroscience; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.8 kDa

Observed Molecular Weight

50-70 kDa

Species

Human