Recombinant Human Glypican-3/GPC3/OCI5 (C-6His-Avi) Biotinylated

Specifications

Catalog number

PKSH033852-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glypican-3/GPC3 is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceeds both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.

Abbreviation

Glypican-3; GPC3; OCI5

Synonyms

CDw329; Protein FOAP-9; SIGLEC9; Sialic acid-binding Ig-like lectin 9; Siglec-9

UniProt

P51654

Accession Number

P51654

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-Avi

Sequence

Gln25-His559

Field of Research

Signal Transduction; Cancer; Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

63.4 kDa

Observed Molecular Weight

60-180&36-44 kDa

Species

Human