Recombinant Human B- and T-Lymphocyte Attenuator/BTLA/CD272 (C-mFc)

Specifications

Catalog number

PKSH033861-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B- and T-Lymphocyte Attenuator (BTLA) is a single-pass type I membrane protein containing 1 Ig-like V-type (immunoglobulin-like) domain. BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 and CTLA4, BTLA interacts with a B7 homolog, B7H4. However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family receptors (TNF-R), not just the B7 family of cell surface receptors. BTLA is a lymphocyte inhibitory receptor that inhibits lymphocytes during immune response. BTLA also is a ligand for tumor necrosis factor (receptor) superfamily, member 14 (TNFRSF14), also known as herpes virus entry mediator (HVEM) . BTLA-HVEM complexes negatively regulate T-cell immune responses.

Abbreviation

BTLA; CD272

Synonyms

B- and T-Lymphocyte Attenuator; B- and T-Lymphocyte-Associated Protein; BTLA; CD272

UniProt

Q7Z6A9

Accession Number

Q7Z6A9-2

Expression System

HEK293 Cells

Tag

C-mFc

Sequence

Lys31-Leu150

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.4 kDa

Observed Molecular Weight

50-70 kDa

Species

Human