Recombinant Human Interleukin-36 alpha/IL-36 alpha

Specifications

Catalog number

PKSH033873-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

Abbreviation

IL-36A; IL-36 alpha

Synonyms

FIL; IL1F; Interleukin-1 Family Member; IL-1F; IL36A; FIL1; FIL1 (EPSILON) ; FIL1E; IL-1F6; IL1 (EPSILON) ; IL1F6; FIL1 Epsilon; IL-1 Epsilon; Interleukin-1 Epsilon; Interleukin-1 Family Member 6; Interleukin-36 Alpha; IL1E; IL 1 epsilon; IL 1F6; IL 1H1; IL1RP2; Interleukin 1 epsilon; Interleukin 1 family member 6; Interleukin 1 family member 6 (epsilon) ; Interleukin 36 alpha; MGC129552; MGC129553; MGC151479; MGC151481; OTTMUSP00000012798; RP23-176J12.4

UniProt

Q9UHA7

Accession Number

Q9UHA7

Expression System

E.coli

Tag

C-His

Sequence

Lys6-Phe158

Applications

Cell culture

Field of Research

Immunology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.1 kDa

Observed Molecular Weight

17 kDa

Species

Human