Recombinant Human CD99/MIC2 (C-6His)

Specifications

Catalog number

PKSH033876-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD99 is a type I transmembrane glycoprotein and the founding member of the CD99 family of molecules. The extracellular domain of CD99 contains no identifiable motifs, its cytoplasmic region, although short, does have signal transduction capability. Cells known to express CD99 include fibroblasts, neutrophils, T cells, double positive thymocytes, CD34+ stem cells, monocytes and endothelial cells. Two types of CD99 isoforms have been classified. Native human CD99 is referred to as the long, or type I isoform. The best studied type II isoform shows an Asp-Gly substitution for the C terminal 27 amino acids. The type I and II isoforms have distinctive signal transduction pathways (FAKsrc for type I PI3K plus srcERK1/2 for type II), and mediate clearly different biological outcomes. Homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell regulates the transendothelial migration of neutrophils during inflammation. Human CD99 has 48% aa sequence identity to mouse CD99.

Abbreviation

CD99; MIC2

Synonyms

12E7; CD99; CD99 Antigen; E2 Antigen; MIC2; MIC2X; MIC2Y; Protein MIC2; T-Cell Surface Glycoprotein E2

UniProt

P14209

Accession Number

P14209

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp23-Asp122

Field of Research

Signal Transduction; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.1 kDa

Observed Molecular Weight

18 kDa

Species

Human