Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (AAH65937.1, C-Fc)

Specifications

Catalog number

PKSH033885-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA) . The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Abbreviation

5'-Nucleotidase;5'-NT; CD73

Synonyms

5'-NT;5'-Nucleotidase; CD73; Ecto-5'-Nucleotidase; NT5; NT5E; NTE

Accession Number

AAH65937.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Trp27-Lys547

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

84.6 kDa

Observed Molecular Weight

85-100 kDa

Formulation

2

Species

Human