Recombinant Human CD300LB/LMIR5 (C-Fc)

Specifications

Catalog number

PKSH033897-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD300LB, also known as CD300b, LMIR5, CLM-7, and IREM-3, is a glycoprotein member of the immunoglobulin superfamily. LMIR5 is expressed on the surface of myeloid lineage cells. It forms noncovalent cis-homodimers and cis-heterodimers with other CD300 family proteins, and the composition of these dimers affects the cellular response. Antibody cross-linking of LMIR5 induces mast cell granule release and cytokine production as well as its tyrosine phosphorylation of LMIR5 (in human) . LMIR5 interacts with TIM1 and TIM4 which regulate T cell activation and are themselves binding partners. TIM1 interactions with LMIR5 mediate mast cell activation and the accumulation of neutrophils at sites of TIM1 up regulation on damaged renal tubule epithelial cells. Acts as an activating immune receptor through its interaction with ITAM-bearing adapter TYROBP, and also independently by recruitment of GRB2.

Abbreviation

CD300LB; LMIR5

Synonyms

CD300LB; CD300b; CLM-7; CMRF35-A2; IREM-3; IREM3; TREM-5; TREM5

UniProt

A8K4G0

Accession Number

AAH28091.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ile55-His187

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42.2 kDa

Observed Molecular Weight

50-60 kDa

Species

Human