Recombinant Human Leukocyte Ig-Like Receptor B2/LILRB2/ILT4/CD85d (C-Fc)

Specifications

Catalog number

PKSH033909-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR) . Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC) . Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.

Abbreviation

LILRB2; ILT4; CD85d

Synonyms

CD85 Antigen-Like Family Member D; CD85d; ILT; ILT-4; Immunoglobulin-Like Transcript 4; LILRB2; LIR-2; Leukocyte Immunoglobulin-Like Receptor 2; Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2; MIR-10; Monocyte/Macrophage Immunoglobulin-Like Receptor 10

Accession Number

AAH36827.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln22-His458

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.5 kDa

Observed Molecular Weight

90-120 kDa

Species

Human