Recombinant Human Siglec-10 (C-Fc)

Specifications

Catalog number

PKSH033913-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Abbreviation

Siglec-10

Synonyms

MGC126774; PRO940; SIGLEC10; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7; Siglec-10; Siglec-like protein 2; Siglec10; sialic acid-binding Ig-like lectin 10; siglec-like gene 2

UniProt

Q96LC7

Accession Number

Q96LC7

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Met17-Thr546

Applications

ELISA

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Anti-Human Siglec10 mAb at 2μg/ml (100 μl/well) can bind Human Siglec-10-Fc. The ED50 of Human Siglec-10-Fc is 9.18 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

85.1 kDa

Observed Molecular Weight

110-120 kDa

Species

Human