Recombinant Human/Cynomolyus CD28/TP44 (C-Fc-Avi) Biotinylated

Specifications

Catalog number

PKSH033948-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-cell-specific surface glycoprotein CD28 (CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin (Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Abbreviation

CD28; TP44

Synonyms

CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; TP44; Tp44

UniProt

P10747

Accession Number

P10747

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-Fc-Avi

Sequence

Asn19-Pro152

Applications

BLI

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Human B7-1-His on HIS1K Biosensor, can bind Biotinylated Human/Cynomolgus CD28-Fc-Avi with an affinity constant of 9.09 pM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.1 kDa

Observed Molecular Weight

60-90 kDa

Species

Human/Cynomolyus