Recombinant Human B-cell Receptor CD22/Siglec-2/CD22 (C-6His) Biotinylated

Specifications

Catalog number

PKSH033956-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

Abbreviation

Siglec-2; CD22

Synonyms

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; CD22 antigenMGC130020; CD22 molecule; SIGLEC2FLJ22814; Siglec-2; T-cell surface antigen Leu-14; sialic acid binding Ig-like lectin 2

UniProt

P20273

Accession Number

P20273

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-Avi

Sequence

Asp20-Arg687

Applications

BLI

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Biotinylated Human Siglec-2-His-Avi on HIS1K Biosensor, can bind Anti-Human Siglec-2 mAb-Fc with an affinity constant of 0.16 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

78.0 kDa

Observed Molecular Weight

110-130 kDa

Species

Human