Recombinant Human Signal Transducer CD24/CD24 (C-Fc)

Specifications

Catalog number

PKSH033968-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.

Abbreviation

CD24

Synonyms

CD24; CD24A; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

UniProt

P25063

Accession Number

P25063

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ser27-Gly 59

Applications

ELISA

Field of Research

Neuroscience; Cancer; immunology; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Anti-Human CD24 mAb at 2μg/ml (100 μl/well) can bind Human CD24-Fc: Biotinylated by NHS-biotin prior to testing. The ED50 of Human CD24-Fc is 7.62 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.8 kDa

Observed Molecular Weight

33-50 kDa

Species

Human