Recombinant Human Wnt3a/Wnt-3a

Specifications

Catalog number

PKSH033972-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity) . Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) .

Abbreviation

Wnt3a; Wnt-3a

Synonyms

MGC119418; MGC119419; MGC119420; Wnt-3a; Wnt3a; member 3A; protein Wnt-3a; wingless-type MMTV integration site family

Expression System

HEK293 Cells

Tag

None

Applications

Cell culture

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

58.5 kDa

Observed Molecular Weight

66-73 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.

Species

Human