Recombinant Human Angiopoietin-related Protein 3/ANGPTL3 (C-Fc)

Specifications

Catalog number

PKSH033975-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N-and C-terminal segments containing the coiled coil domain and fibrinogen-like domains, respectively. ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake. ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TRβ or PPARβ.

Abbreviation

Angiopoietin-related3; ANGPTL3

Synonyms

ANG-5; ANGPT5; ANGPTL3; Angiopoietin-5; Angiopoietin-like protein 3; Angiopoietin-related protein 3

UniProt

Q9Y5C1

Accession Number

Q9Y5C1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ser17-Pro220

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.7 kDa

Observed Molecular Weight

60-70 kDa

Species

Human