Recombinant Human Neuropilin-1/NRP1 (C-Avi-6His) Biotinylated

Specifications

Catalog number

PKSH033988-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. Neuropilin-1 binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors.

Abbreviation

Neuropilin-1; NRP1

Synonyms

BDCA4; CD304; NRP1; NRPNP1; VEGF165R

UniProt

O14786

Accession Number

AAH07533.1

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-Avi-His

Sequence

Phe22-Lys644

Field of Research

Cardiovascular, Neuroscience, Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.5 kDa

Observed Molecular Weight

85-100 kDa

Species

Human