Recombinant Human DC-SIGN/CD209 (N-Fc)

Specifications

Catalog number

PKSH033989-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD209 is also known as CLEC4L, DC-SIGN and CD209 antigen, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4) . DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors.

Abbreviation

DC-SIGN; CD209

Synonyms

CD209; CD209 Antigen; CD209 molecule; CDSIGNHIV gpl20-binding protein; CLEC4L; DC-SIGN; DC-SIGN1; DCSIGN

UniProt

Q9NNX6

Accession Number

Q9NNX6

Expression System

HEK293 Cells

Tag

N-Fc

Sequence

Gln59-Ala404

Field of Research

Signal Transduction; Microbiology; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

65.3 kDa

Observed Molecular Weight

75 kDa

Species

Human