Recombinant Human SLAM Family Member 7/SLAMF7/CD319/CRACC (C-mFc)

Specifications

Catalog number

PKSH033992-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SLAMF7 is a single-pass type I membrane protein and contains 1 Ig-like C2-type (immunoglobulin-like) domain. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM) -associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, SLAMF7 can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.

Abbreviation

SLAMF7; CD319; CRACC

Synonyms

UNQ; PRO; SLAM; SLAMF7;19A; CD319; CRACC; CS1;19A24 protein; CD 319; CD2 like receptor activating cytotoxic cells; CD2 subset 1; CD2-Like Receptor-Activating Cytotoxic Cells; CD319 antigen; Membrane protein FOAP 12; Membrane Protein FOAP-12; Novel Ly9; Novel LY9 (lymphocyte antigen 9) like protein; Protein 19A; SLAM family member 7; SLAM7; SLAMF 7; UNQ576; PRO1138; CS1; CD319; CRACC; CS1; Novel Ly9

UniProt

Q9NQ25

Accession Number

Q9NQ25

Expression System

HEK293 Cells

Tag

C-mFc

Sequence

Ser23-Met226

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.0 kDa

Observed Molecular Weight

60-90 kDa

Species

Human