Recombinant Human SLAM Family Member 5/SLAMF5/CD84 (C-6His-Avi) Biotinylated

Specifications

Catalog number

PKSH034005-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SLAM family member 5 (SLAMF5/CD84) is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function. Mature human CD84 consists of a 204 amino acid (aa) extracellular domain (ECD) with two Iglike domains, a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain with two immunoreceptor tyrosinebased switch motifs (ITSMs) . CD84 exhibits homophilic binding which is mediated by the N-terminal Ig-like domain. Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS/Fli1-activated transcript 2) .CD84 signaling inhibits Fc epsilon RI-induced mast cell activation but enhances platelet activation. LPS-induced macrophage activation, T cell proliferation and IFN-γproduction, and the interactions between T cells and B cells that are required for germinal center formation.

Abbreviation

SLAM

Synonyms

HCD; mCD; SLAM family member; Cell surface antigen MAX; Leukocyte differentiation antigen CD; Signaling lymphocytic activation molecule; SLAMF; Cell surface antigen MAX.3; hCD84; Hly9-beta; Leukocyte differentiation antigen CD84; LY9B; mCD84; Signaling lymphocytic activation molecule 5; SLAM family member 5; SLAMF5; CD84; GR6

UniProt

Q9UIB8

Accession Number

Q9UIB8

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-Avi

Sequence

Lys22-Arg220

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

24.9 kDa

Observed Molecular Weight

40-50 kDa

Species

Human