Recombinant Human TRAIL R1 / DR4 / TNFRSF10A (C-6His)

Specifications

Catalog number

PKSH034011-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF1/TRAIL), and thus transduces cell death signal and induces cell apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF1A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.

Abbreviation

TRAIL R1; DR4; TNFRSF10A

Synonyms

APO2; CD261; CD261 antigen; DR4; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL-R1; TRAILR1; cytotoxic TRAIL receptor

UniProt

O00220

Accession Number

O00220

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala24-Asn239

Field of Research

Cell biology; Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.7 kDa

Observed Molecular Weight

19-30 kDa

Species

Human