Recombinant Human IL-28A protein (His Tag)

Specifications

Catalog number

PKSH034112-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-28A (Interferon-λ2, IFN-λ2), IL-28B/IFN-λ3, and IL-29/IFN-λ1 are type III interferons which are distantly related to IL-10 family and type I IFN family cytokines. Mature human IL-28A is an approximately 22-25 kDa protein that shares 66% amino acid (aa) sequence identity with mouse and rat IL-28A and shows cross-species activity. It shares 96% and 70% aa sequence identity with human IL-28B and IL-29, respectively. IL-28A promotes the Th1 polarization of dendritic cells in the airway and inhibits Th2 and Th17 mediated inflammation. IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses in vivo. In contrast, it is up-regulated in invasive bladder cancer where it promotes tumor cell migration.

Abbreviation

IL-28A

Synonyms

IFN-lambda; Cytokine Zcyto; Interferon lambda; Ifnl; ZCYTO; IFN-λ; Cytokine Zcyto20; IFN-lambda-2; IFN-λ2; IL-28A; Interferon lambda-2; Interleukin-28A; IL28A; ZCYTO20; IFNL2; ZCYTO20

UniProt

Q8IZJ0

Accession Number

Q8IZJ0

Expression System

E.coli

Tag

C-His

Sequence

Pro 27-Val 200

Applications

Cell culture

Field of Research

Immunology, Microbiology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.6 kDa

Observed Molecular Weight

20 kDa

Species

Human