Recombinant Human IL-32 alpha protein (His Tag)

Specifications

Catalog number

PKSH034116-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.

Abbreviation

IL-32 alpha

Synonyms

TAIFa; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; TAIFb; TAIFc; TAIFd; IL32; NK4; TAIF; IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; IL-32; Interleukin 32; Interleukin 32 small; Interleukin 32 theta; Interleukin-32; interleukin-32 eta; Natural killer cell transcript 4; Natural killer cells protein 4; NK 4; OTTHUMP00000236040; OTTHUMP00000236041; OTTHUMP00000236042; OTTHUMP00000236043; OTTHUMP00000236044; OTTHUMP00000236045; OTTHUMP00000236046; OTTHUMP00000236047; OTTHUMP00000236048; OTTHUMP00000236049; OTTHUMP00000236050; OTTHUMP00000236051; OTTHUMP00000236052; OTTHUMP00000236053; OTTHUMP00000236054; OTTHUMP00000241545; OTTHUMP00000241602; OTTHUMP00000241603; OTTHUMP00000241604; OTTHUMP00000241605; OTTHUMP00000241606; OTTHUMP00000241607; OTTHUMP00000241608; OTTHUMP00000241609; OTTHUMP00000241610; OTTHUMP00000241611; OTTHUMP00000241612; Tumor necrosis factor alpha-inducing factor

UniProt

P24001-4

Accession Number

P24001-4

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Lys 131

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.7 kDa

Observed Molecular Weight

18 kDa

Species

Human