Recombinant Human BMP-10 protein (His Tag)

Specifications

Catalog number

PKSH034137-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.

Abbreviation

BMP-10

Synonyms

BMP-10

UniProt

O95393

Accession Number

O95393

Expression System

E.coli

Tag

C-His

Sequence

Asn 317-Arg 100

Applications

Cell culture

Field of Research

Signal Transduction, Cardiovascular

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.1 kDa

Observed Molecular Weight

16 kDa

Species

Human