Recombinant Human TGF beta 1 protein (His Tag)

Specifications

Catalog number

PKSH034147-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

Abbreviation

TGF beta 1

Synonyms

CED; Cartilage-inducing factor; DPD1; Differentiation inhibiting factor; IBDIMDE; LAP; TGFB

UniProt

P01137

Accession Number

P01137

Expression System

HEK293 Cells

Tag

N-His

Sequence

Met 1-Ser 390

Applications

Cell culture

Field of Research

Signal Transduction, Stem Cells, Cancer, Metabolism, Cardiovascular, Cell Biology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measure by its ability to inhibit the lL-4 dependent proliferation in TF-1cells. The ED50 for this effect is < 0.1ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.3 kDa

Observed Molecular Weight

10、40、50 kDa

Formulation

Lyophilized from sterile PBS, pH 7.0 Please refer to the specific buffer information in the printed manual.

Species

Human