Recombinant Human CXCL11 protein (His Tag)

Specifications

Catalog number

PKSH034196-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

Abbreviation

CXCL11

Synonyms

B-R1; I-TAC; Interferon Inducible T-Cell α Chemokine

UniProt

O14625

Accession Number

O14625

Expression System

E.coli

Tag

N-His

Sequence

Phe 22-Phe 94

Applications

Cell culture

Field of Research

Immunology, Neuroscience

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Measure by its ability to chemoattract human THP1 cells using a concentration range of 10-100 ng/mL

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

9.1 kDa

Observed Molecular Weight

11 kDa

Species

Human