Recombinant Mouse PDK4 Protein (His & GST Tag)

Specifications

Catalog number

PKSM040290

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC) .PDK4 gene expression is stimulated by thyroid hormone (T (3) ), glucocorticoids, and long chain fatty acids.

Synonyms

AV005916

UniProt

O70571

Accession Number

O70571

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met1-Val412

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Kinase activity untested

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

74.4 kDa

Observed Molecular Weight

65 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0

Species

Mouse