Recombinant Mouse NEK3 Protein (His & GST Tag)

Specifications

Catalog number

PKSM040293

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NEK3 (NIMA (never in mitosis gene a) -related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a) . NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

Synonyms

NEK3

Accession Number

ABK42288.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Ala 509

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

84.8 kDa

Observed Molecular Weight

72 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol

Species

Mouse